Anti-S. Aureus Recombinant Antibody (TAB-029)

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Great for IP Studies

I used the Anti-S. Aureus Recombinant Antibody (Tefibazumab) in an immunoprecipitation experiment to study the ClfA protein of S. aureus. The antibody's high purity was immediately evident. It worked like a charm, specifically pulling down the target protein without any issues. The fact that it's from the Hi - Affi™ recombinant antibody portfolio with its increased sensitivity was a huge plus. In my IP experiment, I was able to detect the ClfA protein even in samples with a relatively low amount of S. aureus. Considering the related staphylococcal infections, understanding the ClfA protein through IP is crucial, and this antibody delivered excellent results.

FuncS Helper

I used this antibody for a functional study (FuncS) of S. aureus, focusing on the role of the ClfA protein in the bacterium's virulence. The antibody's excellent batch-to-batch consistency was a godsend. I could rely on it to perform the same way in each experiment. Its high sensitivity allowed me to detect even minor changes in the function of the ClfA protein. Considering the related diseases caused by S. aureus, understanding the protein's function is vital, and this antibody was a great tool for my research.

FC Performer

When I used this antibody in a flow cytometry (FC) experiment to analyze the proportion of S. aureus - infected cells in a mixed population, it worked perfectly. The controls were easy to set up, and the antibody's specificity towards S. aureus made it simple to distinguish between infected and non - infected cells. The high purity of the antibody minimized non - specific binding, which was a big concern for me. Given its applications in FC and its relation to staphylococcal infections research, I'm very satisfied with my purchase.

Q&As

1. I'm planning to use this Anti - S. Aureus Recombinant Antibody (Tefibazumab) in an immunofluorescence (IF) experiment to study S. aureus in a cell culture model. Given its high purity, will it bind specifically to the S. aureus ClfA protein without cross - reacting with other proteins in the cell lysate?

   A: This antibody has been confirmed to have high specificity, as it is tested positive against native S. aureus antigen. Its high purity, which is greater than 95% by SDS - PAGE, also reduces the likelihood of cross - reactivity. In an IF experiment, the antibody's specificity towards the ClfA protein of S. aureus should be reliable. However, it's always a good idea to run proper controls, like using a non - specific antibody or pre - absorbing the antibody with the antigen, to ensure that the observed fluorescence is truly due to the binding of Tefibazumab to the ClfA protein and not to other proteins in the cell lysate.

2. I want to use this antibody in an ELISA to detect S. aureus in environmental samples. Considering the related disease being staphylococcal infections, will the antibody be able to detect different strains of S. aureus present in these samples?

   A: The Anti - S. Aureus Recombinant Antibody (Tefibazumab) is reactive against S. aureus. While there are many strains of S. aureus, the antibody is targeted against the ClfA protein which is present in S. aureus. However, different strains may have some variability in the ClfA protein structure. In most cases, the antibody should be able to detect different strains as long as the ClfA protein has a sufficient degree of conservation. It's advisable to test the antibody against known different strains of S. aureus in a preliminary experiment to ensure its effectiveness in detecting the specific strains present in your environmental samples.

3. Can this antibody be used in a flow cytometry (FC) experiment to analyze the proportion of S. aureus - infected cells in a mixed cell population? If so, what controls should I include?

   A: Yes, this Anti - S. Aureus Recombinant Antibody (Tefibazumab) can be used in FC for such an analysis. As for controls, include an unstained sample to set the baseline fluorescence. A sample stained with an isotype - matched control antibody is also essential. This control helps to account for non - specific binding of the antibody. Given the antibody's high specificity towards S. aureus, it should accurately detect the S. aureus - infected cells in the mixed population. However, optimize the antibody concentration and staining conditions to get the best separation between positive and negative populations.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-029, RRID: AB_3111763)

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Biosimilar Overview

Please refer to Tefibazumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tefibazumab.

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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